small extracellular vesicles (sevs) from mesenchymal stromal cell (msc-sevs)  (BioMimetic Therapeutics)

Journal: STAR Protocols

Article Title: Protocol for the isolation and characterization of extracellular vesicles and particles from human and murine cell lines

doi: 10.1016/j.xpro.2026.104505

Figure Lengend Snippet: Cell culture and EVP isolation (A) MHCC97L cells are cultured in standard full medium, cell confluency is checked and maintained at ∼80%. (B) Remove the medium, cells are washed twice with 15 mL sterile 37°C PBS to remove residual serum and medium components. (C) MHCC97L cells are cultured in EVP-depleted full medium for 72 h, with cell confluency and morphology monitored every 24 h; for optimal EVP isolation, cultures are maintained at >95% confluency. (D) Schematic illustrating the isolation of small extracellular vesicles (sEV) and exomeres (EM) from cell-conditioned medium by sequential ultracentrifugation.

Article Snippet: Small extracellular vesicles (sEV; exosome) are lipid membrane-enclosed nanoparticles that are important for intercellular communication by transferring various biological cargoes.

Techniques: Cell Culture, Isolation, Sterility

Journal: STAR Protocols

Article Title: Protocol for the isolation and characterization of extracellular vesicles and particles from human and murine cell lines

doi: 10.1016/j.xpro.2026.104505

Figure Lengend Snippet: Characterization of small extracellular vesicles (sEV) and exomeres (EM) (A) Protein yield (μg per 10 7 cells) of fresh sEV and stored sEV; data are presented as mean ± SEM, P < 0.05 (Student’s t test). (B) Total protein yield (μg per 10 7 cells) of fresh EM and stored EM; data are presented as mean ± SEM, NS (not significant, Student’s t test). (C) Western blot analysis of total cell lysate (TCL), sEV, and EM fractions: exosome positive markers (Alix, TSG101, CD9), negative marker (GM130), and EM-enriched proteins (MAN2B1, GALNS); GAPDH serves as a loading control. (D) Representative transmission electron microscopy (TEM) images of isolated sEV (left) and EM (right); scale bar = 100 nm. (E) Nanoparticle tracking analysis (NTA) of sEV.

Article Snippet: Small extracellular vesicles (sEV; exosome) are lipid membrane-enclosed nanoparticles that are important for intercellular communication by transferring various biological cargoes.

Techniques: Western Blot, Marker, Control, Transmission Assay, Electron Microscopy, Isolation

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: Summary of Nanoparticles for Stroke Treatment by Angiogenesis

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Over Expression, Formulation, In Vitro, Activity Assay, Molecular Weight, Microinjection, Functional Assay, shRNA, Plasmid Preparation, Expressing, Adsorption, Permeability, Comparison, Cell Culture, Membrane, Disruption, Preserving, Liposomes, Derivative Assay, Generated, In Vivo, Control, Knock-Out, Injection

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: iMSC-sEV promote angiogenesis following stroke and increase HUVEC migration and tube formation after OGD. ( A – D ) Seven days after MCAO, angiogenesis was evaluated by immunofluorescence staining of CD31/EdU and CD34. Scale bar = 100 μm. * P < 0.05. Reproduced from Xia Y, Ling X, Hu G, et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther . 2020;11(1):313. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Migration, Immunofluorescence, Staining, Derivative Assay

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: When PMNs are absent in vivo, sEVs produced from hypoxic MSCs promote microvascular remodeling after ischemic stroke. ( A – C ) The microvascular network properties in the ischemic cerebral cortex were examined using 3D light sheet microscopy in mice that were subjected to 40 minutes of MCAO and then survived for 14 days. A, microvascular length density; B, branch point density; and C, mean branch length. ( D ) Magnified axial views of the striatum and cortex in an ischemic mouse brain showing the regions of interest for determining the properties of the microvascular network. Images of the ischemic cortex taken using maximum intensity projection ( E ) from each of the five groups. * p <0.05 compared with isotype/vehicle; # p <0.05 compared with isotype/sEV hypoxic . Scale bars=500 µm (in ( D )/100 µm (in ( E ). Reproduced from Gregorius J, Wang C, Stambouli O, et al. Small extracellular vesicles obtained from hypoxic mesenchymal stromal cells have unique characteristics that promote cerebral angiogenesis, brain remodeling and neurological recovery after focal cerebral ischemia in mice. Basic Res Cardiol . 2021;116(1):40. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: In Vivo, Produced, Microscopy